(DOWNLOAD) "Human Atp-Binding Cassette Transporter Taqman Low-Density Array: Analysis of Macrophage Differentiation and Foam Cell Formation (Technical Briefs)" by Clinical Chemistry ~ Book PDF Kindle ePub Free
eBook details
- Title: Human Atp-Binding Cassette Transporter Taqman Low-Density Array: Analysis of Macrophage Differentiation and Foam Cell Formation (Technical Briefs)
- Author : Clinical Chemistry
- Release Date : January 01, 2006
- Genre: Chemistry,Books,Science & Nature,
- Pages : * pages
- Size : 201 KB
Description
ATP-binding cassette (ABC) transporters cause various human monogenic and polygenic diseases and regulate many physiologic processes, such as lipid homeostasis, iron transport, and immune mechanisms (1). Numerous ABC transporters are involved in bile acid, phospholipid, and sterol transport (2), and expression of the genes that encode these transporters is itself controlled by lipids. Several ABC proteins mediate drug export in compound-treated tumor cells and thereby promote the development of multidrug resistance (3). Thus, ABC transporter mRNA profiling may become an integral part of lipidomics, molecular clinical diagnosis, and monitoring of drug effects in cancer patients (4). We recently described a real-time reverse transcription-PCR method for detection and quantification of 47 of the 48 currently known members of the ABC transporter superfamily (5). This analysis was based on relative quantification using the Standard Curve Method and allowed monitoring of RNA samples in a 384-well format with only 50 ng of total RNA. We have successfully used the method to quantify ABC transporter mRNA in 20 different human tissues.
 "Human Atp-Binding Cassette Transporter Taqman Low-Density Array: Analysis of Macrophage Differentiation and Foam Cell Formation (Technical Briefs)" by Clinical Chemistry ~ Book PDF Kindle ePub Free){kind=link}